KMID : 0854720060260020122
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Korean Journal of Asthma, Allergy and Clinical Immunology 2006 Volume.26 No. 2 p.122 ~ p.128
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Cellular Sources of T-bet in Asthma are Determined by Atopic Status
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Park Chang-Han
Jee Young-Koo Chun Eun-Mi Lee Ji-Soo Cho Young-Joo
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Abstract
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Background: T-bet is considered as a master transcriptional factor that appears to regulate lineage commitment of CD4+ T cells in part by activating the TH1 cytokine IFN-gamma. As the IFN-gamma is also produced by CD8+ T cells, CD56+CD3- NK cells and CD56+CD3+ NKT cells, T-bet may also be expressed in these cells. However, expression and cellular sources of this factor are still unknown in asthmatics.
Objective: Our aim was to investigate the expressions of T-bet in the peripheral blood mononuclear cells (PBMCs) according to presence of atopy and asthma.
Methods: PBMCs were obtained from nonatopic controls, atopic asthmatics and nonatopic asthmatics. Expressions of T-bet according to lymphocytes subtype were analyzed using three-color flow cytometry.
Results: Among the T-bet expressing lymphocytes, percentage of CD3+ T cells and CD56+CD3- NK cells were similar in nonatopic controls. CD3+ T cells were prominent source of T-bet compared to CD56+CD3- NK cells in nonatopic asthmatics (P<0.05). CD56+CD3- NK cells were prominent sources of T-bet compared to CD3+ T cells in atopic asthmatics (P<0.05). The proportion of T-bet positive CD56+CD3+ NKT cells of both controls and nonatopic asthmatics had a high tendency compared to the atopic asthmatics.
Conclusion: This result provides the first evidence about cellular sources of T-bet in PBMCs of asthmatics. The main source of T-bet is different according to presence of atopy.
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KEYWORD
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Asthma, Atopy, NKT cell, T-bet
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